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238 changes: 130 additions & 108 deletions config/robots.rb
Original file line number Diff line number Diff line change
Expand Up @@ -195,11 +195,65 @@
class: 'Robots::PoolingRobot'
)

# Used for ISC and re-ISC pipelines
# Handles both LB Lib PCR-XP and LTN Lib PCR XP (for targeted nanoseq) source plates
# 4:1 pooling into the destination LB Lib PrePool plate
# Replacement robot for nx-8
custom_robot(
'i5-8-lib-pcr-xp-to-isch-lib-pool',
name: 'i5-8 Lib PCR-XP => LB Lib PrePool',
beds: {
bed(5).barcode => {
purpose: ['LB Lib PCR-XP', 'LTN Lib PCR XP'],
states: %w[passed qc_complete],
child: bed(9).barcode,
label: 'Bed 5'
},
bed(6).barcode => {
purpose: ['LB Lib PCR-XP', 'LTN Lib PCR XP'],
states: %w[passed qc_complete],
child: bed(9).barcode,
label: 'Bed 6'
},
bed(7).barcode => {
purpose: ['LB Lib PCR-XP', 'LTN Lib PCR XP'],
states: %w[passed qc_complete],
child: bed(9).barcode,
label: 'Bed 7'
},
bed(8).barcode => {
purpose: ['LB Lib PCR-XP', 'LTN Lib PCR XP'],
states: %w[passed qc_complete],
child: bed(9).barcode,
label: 'Bed 8'
},
bed(9).barcode => {
purpose: 'LB Lib PrePool',
states: %w[pending started],
parents: [bed(5).barcode, bed(6).barcode, bed(7).barcode, bed(8).barcode],
target_state: 'passed',
label: 'Bed 9'
}
},
destination_bed: bed(9).barcode,
class: 'Robots::PoolingRobot'
)

# Deprecating nx-8 robot in favour of the i5-8 version
simple_robot('nx-8') do
from 'LB Lib PrePool', bed(2)
to 'LB Hyb', bed(4)
end

# ISC pipeline
# Beckman i5-8 bed verification
# LB Hyb source to LB Cap Lib destination
# 1:1 transfer
simple_robot('i5-8') do
from 'LB Lib PrePool', bed(5)
to 'LB Hyb', bed(9)
end

bravo_robot do
from 'LB Hyb', bed(4)
to 'LB Cap Lib', car('1,3')
Expand All @@ -210,11 +264,6 @@
to 'LB Cap Lib PCR', car('4,5')
end

simple_robot('nx-96') do
from 'LB Cap Lib PCR', bed(1)
to 'LB Cap Lib PCR-XP', bed(9)
end

custom_robot(
'star-96-post-cap-pcr-purification',
name: 'STAR-96 LB Cap Lib PCR => LB Cap Lib PCR-XP',
Expand Down Expand Up @@ -252,9 +301,13 @@
to 'LB Cap Lib Pool', bed(2)
end

simple_robot('nx-8') do
from 'LB Cap Lib PCR-XP', bed(4)
to 'LB Cap Lib Pool', bed(2)
# ISC pipeline
# Beckman i5-8 bed verification
# LB Cap Lib PCR-XP source to LB Cap Lib Pool destination
# 1:1 transfer
simple_robot('i5-8') do
from 'LB Cap Lib PCR-XP', bed(5)
to 'LB Cap Lib Pool', bed(9)
end

bravo_robot do
Expand All @@ -272,61 +325,6 @@
to 'PF Post Shear XP', car('2,3')
end

custom_robot(
'nx-96-pf-post-shear-to-pf-post-shear-xp',
name: 'nx-96 PF Post-Shear => PF Post-Shear XP',
beds: {
bed(1).barcode => {
purpose: 'PF Post Shear',
states: ['passed'],
label: 'Bed 1'
},
bed(9).barcode => {
purpose: 'PF Post Shear XP',
states: ['pending'],
label: 'Bed 9',
parent: bed(1).barcode,
target_state: 'started'
},
bed(2).barcode => {
purpose: 'PF Post Shear',
states: ['passed'],
label: 'Bed 2'
},
bed(10).barcode => {
purpose: 'PF Post Shear XP',
states: ['pending'],
label: 'Bed 10',
parent: bed(2).barcode,
target_state: 'started'
},
bed(3).barcode => {
purpose: 'PF Post Shear',
states: ['passed'],
label: 'Bed 3'
},
bed(11).barcode => {
purpose: 'PF Post Shear XP',
states: ['pending'],
label: 'Bed 11',
parent: bed(3).barcode,
target_state: 'started'
},
bed(4).barcode => {
purpose: 'PF Post Shear',
states: ['passed'],
label: 'Bed 4'
},
bed(12).barcode => {
purpose: 'PF Post Shear XP',
states: ['pending'],
label: 'Bed 12',
parent: bed(4).barcode,
target_state: 'started'
}
}
)

bravo_robot transition_to: 'started' do
from 'scRNA Stock', bed(4)
to 'scRNA cDNA-XP', car('2,3')
Expand Down Expand Up @@ -2294,51 +2292,6 @@
}
)

custom_robot(
'nx-96-lhr-pcr-1-and-2-to-lhr-xp',
name: 'NX-96 LHR PCR 1 and 2 => LHR XP',
beds: {
bed(1).barcode => {
purpose: 'LHR PCR 1',
states: ['passed'],
label: 'Bed 1',
child: bed(9).barcode
},
bed(2).barcode => {
purpose: 'LHR PCR 2',
states: ['passed'],
label: 'Bed 2',
child: bed(9).barcode
},
bed(3).barcode => {
purpose: 'LHR PCR 1',
states: ['passed'],
label: 'Bed 3',
child: bed(11).barcode
},
bed(4).barcode => {
purpose: 'LHR PCR 2',
states: ['passed'],
label: 'Bed 4',
child: bed(11).barcode
},
bed(9).barcode => {
purpose: 'LHR XP',
label: 'Bed 9',
states: ['pending'],
target_state: 'passed',
parents: [bed(1).barcode, bed(2).barcode]
},
bed(11).barcode => {
purpose: 'LHR XP',
label: 'Bed 11',
states: ['pending'],
target_state: 'passed',
parents: [bed(3).barcode, bed(4).barcode]
}
}
)

bravo_robot transition_to: 'started', require_robot: true do
from 'LHR XP', bed(4)
to 'LHR End Prep', car('1,4')
Expand Down Expand Up @@ -3747,6 +3700,7 @@
}
)

# Deprecating this in favor of the i5 version
custom_robot(
'beckman-rvi-lib-pcr-xp-to-rvi-lib-prepool',
name: 'Beckman RVI Lib PCR XP => RVI Lib PrePool',
Expand Down Expand Up @@ -3787,6 +3741,50 @@
class: 'Robots::PoolingRobot'
)

# RVI pipeline
# Beckman i5-8 bed verification
# RVI Lib PCR XP source to RVI Lib PrePool destination
# 4:1 transfer
custom_robot(
'i5-8-rvi-lib-pcr-xp-to-rvi-lib-prepool',
name: 'i5-8 RVI Lib PCR XP => RVI Lib PrePool',
beds: {
bed(5).barcode => {
purpose: 'RVI Lib PCR XP',
states: %w[passed],
child: bed(9).barcode,
label: 'Bed 5'
},
bed(6).barcode => {
purpose: 'RVI Lib PCR XP',
states: %w[passed],
child: bed(9).barcode,
label: 'Bed 6'
},
bed(7).barcode => {
purpose: 'RVI Lib PCR XP',
states: %w[passed],
child: bed(9).barcode,
label: 'Bed 7'
},
bed(8).barcode => {
purpose: 'RVI Lib PCR XP',
states: %w[passed],
child: bed(9).barcode,
label: 'Bed 8'
},
bed(9).barcode => {
purpose: 'RVI Lib PrePool',
states: %w[pending],
parents: [bed(5).barcode, bed(6).barcode, bed(7).barcode, bed(8).barcode],
target_state: 'passed',
label: 'Bed 9'
}
},
destination_bed: bed(9).barcode,
class: 'Robots::PoolingRobot'
)

custom_robot(
'bravo-rvi-hyb-to-rvi-cap-lib',
name: 'Bravo RVI Hyb => RVI Cap Lib',
Expand Down Expand Up @@ -3856,6 +3854,7 @@
}
)

# Deprecating in favour of the i5 version
custom_robot(
'beckman-rvi-cap-lib-pcr-xp-to-rvi-cap-lib-pool',
name: 'Beckman RVI Cap Lib PCR XP => RVI Cap Lib Pool',
Expand All @@ -3875,6 +3874,29 @@
}
)

# RVI pipeline
# Beckman i5-8 bed verification
# RVI Cap Lib PCR XP source to RVI Cap Lib Pool destination
# 1:1 transfer
custom_robot(
'i5-8-rvi-cap-lib-pcr-xp-to-rvi-cap-lib-pool',
name: 'i5-8 RVI Cap Lib PCR XP => RVI Cap Lib Pool',
beds: {
bed(5).barcode => {
purpose: 'RVI Cap Lib PCR XP',
states: ['passed'],
label: 'Bed 5'
},
bed(9).barcode => {
purpose: 'RVI Cap Lib Pool',
states: ['pending'],
label: 'Bed 9',
parent: bed(5).barcode,
target_state: 'passed'
}
}
)

# scRNA pipeline
# Hamilton STAR bed verification
# LRC PBMC Defrost PBS 1ml to LRC PBMC Pools
Expand Down
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